果聚糖检测试剂盒 Fructan Assay Kit

果聚糖检测试剂盒 Fructan Assay Kit
商品说明:
产品名称:果聚糖检测试剂盒
英文名称:Fructan Assay Kit
型号规格:100次
品牌:爱尔兰Megazyme公司
果聚糖广泛分布于植物界。单子叶植物,双子叶植物和绿藻中均存在。
以分子结构和分子重量区别果聚糖。也可以分为三类:菊粉组,果聚糖组和带支链组。菊粉组主要或专门由果糖基果糖键组成。果聚糖组主要或专门由果糖基果糖键组成。带支链组由大量的(2→1)和(2→6)果糖基果糖键共同组成。
目前存在很多测定植物材料和食品中果聚糖的方法。被广泛接受的是水解为D-果糖(和D-葡萄糖)后再测定的方法。这存在将蔗糖,D-果糖和D-葡萄糖分别去除或测定的问题,Pontis(1966)已经报道了蔗糖,D-葡萄糖和D-果糖的去除方法,利用蔗糖酶水解蔗糖,再通过氢氧化钠煮沸破坏随之产生的D-果糖和D-葡萄糖及原有的单糖。据报道,蔗糖酶对菊粉组低聚果糖的作用是缓慢的,可以通过选择正确的孵育时间使其失效。通过测定目前使用的纯化的酵母蔗糖酶,发现很难。
但是,本试剂盒提供的方法操作简单,使用标准实验室设备,并且是精确性,可重复性和专一性都很好。采用的酶是高纯度酶,专一性水解蔗糖,淀粉和果聚糖。采用的蔗糖酶快速水解蔗糖但对1-蔗果三糖和其他果聚糖的水解活性可以忽略不计(McCleary和Blakeney,1999)(图2)。在底物浓度为10 mg/mL时,蔗糖与1-蔗果三糖的相对水解比率为3,800:1。
原理:
蔗糖被蔗糖酶水解为D-葡萄糖和D-果糖。同时,如果样品中存在淀粉和麦芽多糖通过高纯β-淀粉酶,普鲁兰酶和麦芽糖酶联合作用被水解D-葡萄糖。用碱性***物处理生成的还原糖,将其继续被还原为糖醇。用稀释的乙酸将溶液调至中性,并去除过量的***物。果聚糖被纯化果聚糖酶(菊粉外切酶)水解为D-葡萄糖和D-果糖,通过PAHBAH法测定生成的还原糖。这个方法方便操作,D-葡萄糖和D-果糖的对应颜色一致。含有半乳糖苷蔗糖寡糖的样品如(棉子糖)在蔗糖/淀粉酶混合物(酶溶液1)处理前推荐使用α-半乳糖苷酶(来源于黑曲霉)孵育。释放的单糖通过碱性***物处理去除。如果处理中包括这一步骤,需要在计算中考虑体积的变化(如溶液5的最终体积应用1.15mL代替1.1mL).
The Fructan test kit is suitable for the specific measurement and analysis of fructan in plant extracts and food products containing starch, sucrose and other sugars.
Colourimetric method for the determination of Fructan in plant
products, foodstuffs and other materials

Principle:
(sucrase)
(1) Sucrose + H2O → D-glucose + D-fructose
(β-amylase + maltase + pullulanase)
(2) Starch + maltosaccharides + H2O → D-glucose
(borohydride)
(3) D-Glucose + D-fructose → D-sorbitol + D-mannitol
(non-reducing)

(exo-inulinase + endo-inulinase)
(4) Fructan + H2O → D-glucose + D-fructose
(100°C, 6 min)
(5) D-Glucose + D-fructose + PAHBAH → PAHBAH colour complex
Kit size: 100 assays
Method: Spectrophotometric at 410 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Flours, plant materials (e.g. onion), food products and other materials
Method recognition:
AOAC (Method 999.03), AACC (Method 32-32.01) and CODEX
(Type III Method)
Advantages

  • Very cost effective
  • All kit reagents stable for > 2 years after preparation
  • Unaffected by high sucrose / reducing sugar concentrations
  • Fructan kits are only available from Megazyme
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Megazyme检测试剂盒-现货

Megazyme检测试剂盒-现货

Megazyme是 一家全球性公司,专注于开发和提供用于饮料、谷物、乳制品、食品、饲料、发酵、生物燃料和葡萄酒产业用的分析试剂、酶和检测试剂盒。Megazyme的许 多检测试剂盒产品已经为众多官方科学协会(包括AOAC, AACC , RACI, EBC和ICC等),经过严格的审核,批准认证为官方标准方法,确保以准确、可靠、定量和易于使用的测试方法,满足客户的质量诉求。
Megazyme的主要产品线包括:
Megazyme检测试剂盒产品   ◆ 检测试剂盒
◆ 酶
◆ 酶底物
◆ 碳水化合物
◆ 化学品/仪器
检测试剂盒特色产品:

货号 中文品名 用途
K-ACETAF 乙酸[AF法]检测试剂盒 酶法定量分析乙酸最广泛使用的方法
K-ACHDF 可吸收糖/膳食纤维检测试剂盒 酒精沉淀法测定膳食纤维
K-AMIAR 氨快速检测试剂盒 用于包括葡萄汁、葡萄酒以及其它食品饮料样品中氨含量的快速检测分析。
K-AMYL 直链淀粉/支链淀粉检测试剂盒 谷物淀粉和而粉中直链淀粉/支链淀粉比例和含量检测
K-ARAB 阿拉伯聚糖检测试剂盒 果汁浓缩液中阿拉伯聚糖的检测
K-ASNAM L-天冬酰胺/L-谷氨酰胺和氨快速检测试剂盒 用于食品工业中丙烯酰胺前体、细胞培养基、以及上清液组分中、L-天冬酰胺,谷氨酰胺和氨的检测分析
K-ASPTM 阿斯巴甜检测试剂盒 专业用于测定饮料和食品中阿斯巴甜含量,操作简单
K-BETA3 β-淀粉酶检测试剂盒 适用于麦芽粉中β-淀粉酶的测定
K-BGLU 混合键β-葡聚糖检测试剂盒 测定谷物、荞麦粉、麦汁、啤酒及其它食品中混合键β-葡聚糖(1,3:1,4-β-D-葡聚糖)的含量
K-CERA α-淀粉酶检测试剂盒 谷物和发酵液(真菌和细菌)中α-淀粉酶的分析测定
K-CITR 柠檬酸检测试剂盒 快速、可靠地检测食品、饮料和其它物料中柠檬酸(柠檬酸盐)含量
K-DLATE 乳酸快速检测试剂盒 快速、特异性检测饮料、肉类、奶制品和其它食品中L-乳酸和D-乳酸(乳酸盐)含量
K-EBHLG 酵母β-葡聚糖酶检测试剂盒 用于测量和分析酵母中1,3:1,6?-β-葡聚糖,也可以检测1,3-葡聚糖
K-ETSULPH 总亚硫酸检测试剂盒 测定葡萄酒、饮料、食品和其他物料中总亚硫酸含量(按二氧化硫计)的一种简单,高效,可靠的酶法检测方法
K-FRGLMQ D-果糖/D-葡萄糖[MegaQuant法]检测试剂盒 适用于使用megaquant?色度计(505nm下)测定葡萄、葡萄汁和葡萄酒中D-果糖和D-葡萄糖的含量。
K-FRUC 果聚糖检测试剂盒 含有淀粉、蔗糖和其他糖类的植物提取物和食品中果聚糖的含量测定。
K-FRUGL D-果糖/D-葡萄糖检测试剂盒 对植物和食品中果糖或葡萄糖含量的酶法紫外分光测定。
K-GALM 半乳甘露聚糖检测试剂盒 食品和植物产品中半乳甘露聚糖的含量检测
K-GLUC D-葡萄糖[GOPOD]检测试剂盒 谷物提取物中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUHK D-葡萄糖[HK]检测试剂盒 植物和食品中D-葡萄糖的含量测定,可以和其它Megazyme检测试剂盒联合使用。
K-GLUM 葡甘聚糖检测试剂盒 植物和食品中葡甘聚糖的含量测定。
K-LACGAR 乳糖/D-半乳糖快速检测试剂盒 用于快速检测食品和植物产品中乳糖、D-半乳糖和L-阿拉伯糖
K-LACSU 乳糖/蔗糖/D-葡萄糖检测试剂盒 混合面粉和其它物料中蔗糖、乳糖和D-葡萄糖的测定
K-LACTUL 乳果糖检测试剂盒 特异性、快速和灵敏测量奶基样品中乳果糖含量
K-MANGL D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 适合测定植物产品和多糖酸性水解产物中D-甘露糖含量
K-MASUG 麦芽糖/蔗糖/D-葡萄糖检测试剂盒 在植物和食品中麦芽糖,蔗糖和葡萄糖的含量检测
K-PECID 胶质识别检测试剂盒 食品配料中果胶的鉴别
K-PHYT 植酸(总磷)检测试剂盒 食品和饲料样品植酸/总磷含量测量的简便方法。不需要通过阴离子交换色谱对植酸纯化,适合于大量样本分析
K-PYRUV 丙酮酸检测试剂盒 在啤酒、葡萄酒、果汁、食品和体液中丙酮酸分析
K-RAFGA 棉子糖/D-半乳糖检测试剂盒 快速测量植物材料和食品中棉子糖和半乳糖含量
K-RAFGL 棉子糖/蔗糖/D-半乳糖检测试剂盒 分析种子和种子粉中D-葡萄糖、蔗糖、棉子糖、水苏糖和毛蕊花糖含量。通过将棉子糖、水苏糖和毛蕊花糖酶解D-葡萄糖、D-果糖和半乳糖,从而测定葡萄糖含量来确定?
K-SUCGL 蔗糖/D-葡萄糖检测试剂盒 饮料、果汁、蜂蜜和食品中蔗糖和葡萄糖的分析
K-SUFRG 蔗糖/D-果糖/D-葡萄糖检测试剂盒 适用于植物和食品中蔗糖、D-葡萄糖和D-果糖的测定
K-TDFR 总膳食纤维检测试剂盒 总膳食纤维检测
K-TREH 海藻糖检测试剂盒 快速、可靠地检测食品、饮料和其它物料中海藻糖含量
K-URAMR 尿素/氨快速检测试剂盒 适用于水、饮料、乳制品和食品中尿素和氨的快速测定
K-URONIC D-葡萄糖醛酸/D-半乳糖醛酸检测试剂盒 简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中六元糖醛酸含量(D-葡萄糖醛酸和D-半乳糖醛酸)
K-XYLOSE D-木糖检测试剂盒 简单、可靠、精确测定植物提取物、培养基/上清液以及其它物料中D-木糖含量
K-YBGL Beta葡聚糖[酵母和蘑菇]检测试剂盒 检测酵母和蘑菇制品中1,3:1,6-beta-葡聚糖和α-葡聚糖含量

麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit

麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit
货号: K-MALTA
品名:麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit
型号:100 assays per kit
α-淀粉酶,β-淀粉酶在淀粉降解代谢过程中或发酵糖发芽或谷物麦芽中发挥重要作用。
上海子起生物提供麦芽淀粉酶检测试剂盒,比色法(400nm)测定谷物、麦芽、食品、饮料和发酵产品中的α-淀粉酶和Beta-淀粉酶。
原理:(说明书 请致电 400-811-9081)
(1) α-淀粉酶检测利用“Ceralpha” 方法
(2) Beta-淀粉酶检测利用 “Betamyl-3” 方法
反应时间:
麦芽淀粉酶检测试剂盒 K-MALTA 
~ 20 min (Ceralpha 方法)
~ 10 min (Betamyl-3 方法)
检测限:0.05 U/mL
适用样品:面粉、麦芽、发酵液和其他原料
优点:
价格低廉(每次检测成本)
所有试剂配制后的稳定性>2年
只适用于酶法试剂盒
特异性高
方法简单
包含标准品
方法认证:AOAC (Method 2002.01), AACC (Method 22-02.01),ICC (Standard No. 303), RACI (Standard Method), and CCFRA (Flour Testing Working Group Method 0018)
RACI (标准方法)
 

纤维素酶检测试剂盒

纤维素酶检测试剂盒[CellG5法], Cellulase Assay Kit (CellG5 Method)

货号:K-CellG5
中文品名:纤维素酶检测试剂盒[CellG5法]
品名:Cellulase Assay Kit (CellG5 Method)
规格1:K-CellG5-4V 120/240次(手工)/480次(自动分析仪)
规格2:K-CellG5-2V 60/120次(手工)/240次(自动分析仪)
说明:CellG5检测试剂盒用于检测纤维素内切酶(1,4 -β-葡聚糖内切酶)包含两种组分:1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2)热稳定的β-葡萄糖苷酶。 酮阻断基团可阻止β-葡萄糖苷酶对BPNPG5的水解。通过纤维素内切酶的培养可生成非阻断的显色寡糖,被β-葡萄糖苷酶迅速水解。产生4-硝基苯酚的速率与β-葡萄糖苷酶水解BPNPG5直接相关。加入Tris缓冲液(pH 9.0)后可终止反应,并形成苯酚显色产物。
CellG5检测法代表了代表了纤维素酶测量方法的巨大进步。传统的纤维素酶检测依赖于酶底物,例如羧甲基纤维素,微晶纤维素,纤维低聚糖,CMC滤纸,染色多糖,包括CMC刚果红或纤维素天青。
应用:纤维素酶检测试剂盒[CellG5法]。

原理:
比色法测定酶制剂、发酵产品中1,4 -β-葡聚糖内切酶(纤维素酶)的含量。
纤维素酶检测试剂盒 (K-CellG5)检测原理
检测方法:分光光度法 @400 nm
反应时间:10min
检测限:3.5 x 10-4 U/mL
应用案例:
发酵液,工业酶制剂和生物燃料研究。
方法识别:
新方法
试剂盒组成:
Bottle 1: (x2) 或 (x4) 每瓶含有4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5)溶于3毫升含有0.02% w/v叠氮钠的10% DMSO水溶液。
-20°C下稳定超过4年。
Bottle 2: (x 2) 热稳定的β-葡萄糖苷酶(0.50 mL, 600 U/mL)溶于含有0.02% w/v叠氮钠的 50% w/v 硫酸铵溶液。
4°C下稳定超过4年。
Bottle 3: 木霉纤维素酶标准溶液 (5 mL, ~ 3 U/mL; 准确至见瓶标签) 溶于含有0.02% w/v叠氮钠的50% 的丙三醇水溶液。
-20°C下稳定超过4年。
优点:

所有试剂可稳定超过4年
价格非常具有竞争力
完全针对纤维素酶(1,4-葡聚糖内切酶)
常规操作,非常灵敏
检测方法十分简单,适合仪器分析
包含标准
参考文献:
Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase). McCleary, B. V., Mangan, D., Daly, R., Fort, S., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 385, 9-17.
Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase. Mangan, D., McCleary, B. V., Liadova, A., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 395, 47-51.
A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase). Mangan, D., Cornaggia, C., McKie, V., Kargelis. T. & V. McCleary, B. V. (2016). Analytical and Bioanalytical Chemistry, 408(15), 4159-4168.
说明书下载地址:https://secure.megazyme.com/files/Booklet/K-CellG5_DATA.pdf

Beta葡聚糖酶[麦芽和微生物]检测试剂盒

Beta葡聚糖酶[麦芽和微生物]检测试剂盒

英文名:β-Glucanase Assay Kit (Malt and Microbial)

货号:K-MBGL

规格:100 assays per kit

     β-葡聚糖酶作用方式不同,可分为内切型和外切型。前者存在于谷物种子、某些真菌和某些细菌中,能催化水解谷物细胞壁中的β-葡聚糖,其中包括内切型β-1,4-葡聚糖酶、内切型β-1,3-葡聚糖酶。后者存在于谷物种子中,其中又包括外切型β-1,4-葡聚糖酶、外切型β-1,3-葡聚糖酶。
在工业上采用细菌(如枯草芽孢杆菌)、曲霉(如黑曲霉)、青霉(如伊氏青霉)生产。在啤酒生产原料大麦及其麦芽中,含本品较多。在啤酒生产中采用β-葡聚糖酶,可提高麦汁的过滤速度和得率,从而保证啤酒质量。也可用于饴糖、麦芽糖浆生产和用作饲料添加剂。

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.
Colourimetric method for the determination of β-Glucanase
in malt, foodstuffs and fermentation products
Principle:
(β-glucanase)
(1) Azo-Barley β-glucan (polymer) → Azo-barley β-glucan
(fragments)

(2) Add alcohol; centrifuge to remove polymeric Azo-barley
β-glucan

(3) Measure the absorbance of the supernatant solution
Kit size: 100 assays
Method: Spectrophotometric at 590 nm
Reaction time: ~ 30 min
Detection limit: 100 U/kg of malt
Application examples:
Malt extracts, wort, beer and other materials
Method recognition:
RACI (Standard Method)
Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only kit available
  • Very specific
  • Simple format
  • Standard included

一级氨基氮[NOPA]检测试剂盒

一级氨基氮[NOPA]检测试剂盒 Primary Amino Nitrogen Assay Kit (PANOPA)

名称:一级氨基氮[NOPA]检测试剂盒
英文名:Primary Amino Nitrogen(NOPA) Assay Kit
规格:100次
产地:爱尔兰
Primary Amino Nitrogen (PANOPA)
For the assay of primary amino nitrogen in grape juice/must and wine.
Catalogue Number: K-PANOPA
Content: 100 assays per kit

商品说明:
The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of Primary Amino Nitrogen in
grape juice, must, wine and other materials
Principle:
(room temperature)
(1) Amino nitrogen + N-acetyl-L-cysteine + o-phthaldialdehyde →
isoindole derivative
Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (autoanalyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.59 mg N/L
Application examples:
Grape juice, must, wine and other materials
Method recognition: Novel method
Advantages

  • Simple format (absorbances read at 340 nm)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

蔗糖/D-果糖/D-葡萄糖检测试剂盒

蔗糖/D-果糖/D-葡萄糖检测试剂盒 Sucrose/Fructose/D-Glucose Assay Kit

产品名称:蔗糖/D-果糖/D-葡萄糖检测试剂盒

英文名称:Sucrose,D-fructose and D-glucose sucrose assay kit

货号:K-SUFRG

型号规格:150 (50 of each)

优点:价格低廉(每次检测成本);

所有试剂配制后的稳定性〉12个月;

反应快;葡萄糖氧化酶/过氧化酶/己糖激酶/6-磷酸葡萄糖脱氢酶;

包含标准品

检测原理:


检测方法:紫外分光光度法

检测波长:340nm;

反应时间:30min;

检测限:1.38mg/L;

适用样品:啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、面包、焙烤产品、乳制品、糖果、甜点、糖食、冰淇淋、水果和蔬菜(如:马铃薯)、肉制品(如香肠)、调味品、饲料、烟、化妆品、纸及其他原料(如生物培养、样品等);

方法认证:EN。NEN。NF。DIN,GOST,IFU ,AIJN,MEI,IOCCC以及德国、奥地利认证

商品说明:
The Sucrose/Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials

Principle:
(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose
(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(5) F-6-P → G-6-P
Kit size: 100 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 1.38 mg/L
Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread,
bakery products, dairy products, candies, desserts, confectionery, sweets,
ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage),
condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics,
pharmaceuticals, paper and other materials
Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN,
DIN, GOST, IFU, AIJN, MEBAK and IOCCC
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Stabilised D-glucose / D-fructose standard solution included
  • Extended cofactors stability

L-精氨酸/尿素/氨检测试剂盒[快速]

L-精氨酸/尿素/氨检测试剂盒[快速] L-Arginine/Urea/Ammonia (Rapid)
产品货号:K-LARGE
品牌:进口
中文名:L-精氨酸/尿素/氨[快速]检测试剂盒
英文名:Integrated Total Dietary Fibre Assay Kit
规格:150 assays

商品说明:
For the specific and rapid assay of L-arginine, urea and ammonia in grape juice/must and wine. Content:150 assays – 50 of each component
UV-method for the determination of L-Arginine, Urea and Ammonia 
in grape juice, must and wine

Principle:
                            (microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+ + 
H2O
                     (urease)
(2) Urea + H2O → 2 NH3 + CO2
(arginase)
(3) L-Arginine + H2O → urea + ornithine

Kit size:                             50 assays
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 20 min [ammonia (2 min), urea (6 min),
L-arginine (7 min)] Detection limit:                  0.07 mg/L (ammonia)
0.13 mg/L (urea)
0.37 mg/L (L-arginine)
Application examples:
Grape juice, wine must, wine and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:          Improved method
Advantages

  • Improved assay format
  • Very rapid reactions due to use of uninhibited glutamate dehydrogenase
  • All enzymes supplied as stabilised suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability

索取产品说明书请联系:18301939375  QQ:3259632176

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 D-mannose/D-fructose/D-glucose assay kit

产品名称:D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

英文名称:D-mannose/D-fructose/D-glucose assay kit

货号: K-MANGL

型号规格:55次

优点:成本低(每次检测成本);所有试剂配制后的稳定性〉2年;只有酶试剂盒可用;方法简单;反应快;包含标准品

检测原理:

检测方法:紫外分光光度法

检测波长:340nm;

反应时间:25min;

检测限:0.66mg/L;

适用样品:食品、酵母细胞制剂、酶水解产物和其他原料(如:生物培养、样品等);

方法认证:新方法

商品说明:
The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.
UV-method for the determination of D-Mannose, D-Fructose
and D-Glucosein foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphomannose isomerase) (phosphoglucose isomerase)
(3) M-6-P ↔ F-6-P ↔ G-6-P

Kit size: 55 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

L-苹果酸检测试剂盒

L-苹果酸检测试剂盒L-Malic Acid Assay Kit (Manual Format)

产品货号: K-LMALL
产品品名: L- 苹果酸检测试剂盒(大)
英文品名: L-Malic Acid Assay Kit (Large)
规格型号: 116 assays
原理:
反应时间: 3min
检测限: 0.25 mg/L,
适用样品: 饮料和食品以及其他原料
优点:
l    成本低
l    使用期间所有试剂的稳定性 >2 年
l    操作简单
l    包含标准品
REFERENCES:

  1. Mollering, H. (1985). L-Malate. In  Methods of Enzymatic Analysis  (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 39-47, VCH Publishers (UK) Ltd., Cambridge, UK.
  2. AOAC Official Methods of Analysis (2002). Method 993.05 “L-Malic acid/total malic acid ratio in apple juice”. 17th ed. Chapter 37, p. 15.
  3. Gorin, N. (1976). Differences in L-malate determined enzymatically or titrimetrically in golden delicious apples.  Zeitschrift fur Lebensmittel-Unterschung und-Forschung,  162, 259-261.
  4. Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer.  J. Inst. Brew.  92, 225-228.
  5. Elkins, E. R. & Freund, W. (1994). Detection of adulteration in apple juice by L-malic/total malic acid ratio: Collaborative Study.  J. AOAC Int . 77, 411-415.
商品说明:
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format