L-苹果酸检测试剂盒L-Malic Acid Assay Kit (Manual Format)
产品货号： K-LMALL
产品品名： L- 苹果酸检测试剂盒（大）
英文品名： L-Malic Acid Assay Kit (Large)
规格型号： 116 assays
原理：
反应时间： 3min
检测限： 0.25 mg/L,
适用样品： 饮料和食品以及其他原料
优点：
l 成本低
l 使用期间所有试剂的稳定性 >2 年
l 操作简单
l 包含标准品
REFERENCES:
- Mollering, H. (1985). L-Malate. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 39-47, VCH Publishers (UK) Ltd., Cambridge, UK.
- AOAC Official Methods of Analysis (2002). Method 993.05 “L-Malic acid/total malic acid ratio in apple juice”. 17th ed. Chapter 37, p. 15.
- Gorin, N. (1976). Differences in L-malate determined enzymatically or titrimetrically in golden delicious apples. Zeitschrift fur Lebensmittel-Unterschung und-Forschung, 162, 259-261.
- Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer. J. Inst. Brew. 92, 225-228.
- Elkins, E. R. & Freund, W. (1994). Detection of adulteration in apple juice by L-malic/total malic acid ratio: Collaborative Study. J. AOAC Int . 77, 411-415.
商品说明：
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
Advantages
Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
Advantages
- PVP incorporated to prevent tannin inhibition
- Both enzymes supplied as stable suspensions
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Very rapid reaction (~ 3 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual and microplate format