Lumiprobe 水溶性Cy染料
磺化和非磺化染料都可用于标记生物分子例如DNA和蛋白质。染料之间的差异是它们的溶解性：磺基染料是水溶性的，并且它们不使用有机共溶剂用于在水性环境中的标记。它们不易于在水中聚集。
There are two varieties of cyanine dyes: non-sulfonated cyanines, and sulfonated cyanines. For many applications they are interchangeable, because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used for the labeling of biomolecules such as DNA and proteins. The difference between the dyes is their solubility: sulfo- dyes are water-soluble, and they do not use of organic co-solvent for the labeling in aqueous environment. They are less prone to aggregation in water.
- Sulfo-Cyanine3 carboxylic acid
- Sulfo-Cyanine5 carboxylic acid
- Sulfo-Cyanine5.5 carboxylic acid
- Sulfo-Cyanine7 carboxylic acid
- Sulfo-Cyanine7.5 carboxylic acid
磺酸基-Cy5羧酸
无活性的磺酸基-Cy5羧酸，水溶性染料。此染料是高水溶性染料
用于分子标记应该选择具有活性的磺酸基-Cy5 NHS酯
分子结构：
一般性质：
外观：深蓝色粉末
分子量：664.76
分子式：C32H37N2NaO8S2
溶解性：易溶于水，DMSO,DMF，在多数有机溶剂难溶
质控：NMR 1H（95%）和13C,TLC
光谱性质：
最大激发波长：646nm
在最大激发波长下的消光系数：271000Lmol-1cm-1
最大发射波长：662nm
荧光量子产率：0.2
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 664.76 |
| CAS number: | 1121756-16-8, 1144162-77-5 |
| Molecular formula: | C32H37N2NaO8S2 |
| Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| Cat. # | Quantity | Lead time |
| 13390 | 1 mg | in stock |
| 23390 | 5 mg | in stock |
| 43390 | 25 mg | in stock |
| 53390 | 50 mg | in stock |
| 63390 | 100 mg | in stock |
磺酸基-Cy7羧酸
磺酸基-Cy7羧酸是无活性易溶于水的近红外染料；
此试剂不能用于标记分子，它有较高的水溶性，可改善近红外光谱范围的量子产率,并具有很高的消光系数。
分子结构：
一般性质：
外观：深绿色粉末
分子量：730.87
分子式：C37h33N2NaO8S2
溶解性：易溶于水，DMF,DMSO
质控：HPLC（95%），NMR 1H 和13C
光谱性质：
最大激发波长：740nm
在最大激发波长下的消光系数：240600Lmol-1cm-1
最大发射波长：773nm
General properties
| Appearance: | dark green powder |
| Molecular weight: | 730.87 |
| Molecular formula: | C37H43N2NaO8S2 |
| Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
| Quality control: | NMR 1H, HPLC (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
Sulfo-Cyanine7 carboxylic acid is non-reactive water soluble near infrared dye.
The reagent is useful as a fluorescent marker in NIR range when attachment to other molecules is not desired. It has high hydrophilicity and aqueous solubility, improved quantum yield in NIR range, and very high molar extinction coefficient.
This reagent contains free carboxylic acid function. Pre-activated activated ester – sulfo-Cyanine7 NHS ester is also available.
| 15390 | 1 mg | in stock |
| 25390 | 5 mg | in stock |
| 45390 | 25 mg | in stock |
| 55390 | 50 mg | in stock |
| 65390 | 100 mg | in stock |
磺基-Cy3 NHS酯：
此染料可溶于水，氨基活性Cy3染料。在水溶液中有效标记蛋白和多肽，无需共溶剂，是低溶解性蛋白和易于降解蛋白的理想选择。
此染料是磺化，亲水性染料。可以代替Alexa Fluor 546和DyLight 549
分子结构：
一般性质：
外观：深红色晶体
分子量：735.8
分子式：C34H38N3NaO10S2
溶解性：极易溶于水，易溶于有机溶剂（DMF,DMSO）
质控：NMR 1H（95%）和13C,TLC，功能性测试
光谱性质：
最大激发波长：548nm
最大激发波长下的消光系数：162000Lmol-1cm-1
最大发射波长：563nm
荧光量子产率：0.1
General properties
| Appearance: | dark red crystals |
| Molecular weight: | 735.80 |
| Molecular formula: | C34H38N3NaO10S2 |
| Solubility: | very high in water, good in polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 11320 | 1 mg | in stock |
| 21320 | 5 mg | in stock |
| 41320 | 25 mg | in stock |
| 51320 | 50 mg | in stock |
| 61320 | 100 mg | in stock |
磺基-Cy5 NHS酯：
水溶性Cy5 NHS酯，用于标记生物分子的氨基，无需任何共溶剂，因此可以用于标记在有机溶剂中易降解的蛋白和溶解性低的蛋白
Cy5 是最常用的荧光团之一，与多种设备兼容，比如平板读数仪，显微镜和成像仪
此染料是高水溶性，可以代替多种用途的Alexa Fluor 647,DyLight 649
分子结构：
一般性质：
外观：深蓝色粉末
分子量：761,84
分子式：C36H40N3NaO10S2
溶解性：极易溶于水，易溶于有机溶剂DMF和DMSO
质量控制：NMR 1H （95%）和13C,TLC,功能性测试
光谱性质：
最大激发波长：646nm
最大激发波长下的消光系数：271000Lmol-1cm-1
最大发射波长：662nm
荧光量子产率：0.2
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| Molecular formula: | C36H40N3NaO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 13320 | 1 mg | in stock |
| 23320 | 5 mg | in stock |
| 43320 | 25 mg | in stock |
| 53320 | 50 mg | in stock |
| 63320 | 100 mg | in stock |
磺基-Cy7 NHS酯：
一般性质：
外观：深绿色粉末
分子量：827.94
分子式：C41H46N3NaO10S2
溶解性：易溶于水,DMF,DMSO
质控：NMR 1H(95%)13C,TLC,功能测试
光谱性质：
最大激发波长：740nm
在最大激发波长下的消光系数：240600Lmol-1cm-1
最大发射波长：773nm
General properties
| Appearance: | dark green powder |
| Mass spec M+ increment: | 868.2 |
| Molecular weight: | 869.10 |
| Molecular formula: | C43H49KN4O9S2 |
| IUPAC name: | 1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt |
| Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 15380 | 1 mg | in stock |
| 25380 | 5 mg | 5 days |
| 45380 | 25 mg | in stock |
| 55380 | 50 mg | in stock |
Sulfo-Cyanine5 maleimide 磺基-cy5-马来酰亚胺
水溶的亲水的sulfo-Cyanine5 maleimide（Cy5® maleimide的类似物）。作为Cy5® maleimide的完美的替代物，我们推荐该产品用于蛋白、包括抗体的标记。通过凝胶过滤、离心柱纯化、透析、电泳或色谱层析方法就可以很容易的将标记蛋白和未标记的染料进行分类。
没有磺化基团的类似物：Cyanine5 maleimide。
磺酸基类荧光染料：
- Sulfo-Cyanine3 NHS ester
- Sulfo-Cyanine5 NHS ester
- Sulfo-Cyanine5 bis-NHS ester
- Sulfo-Cyanine5.5 NHS ester
- Sulfo-Cyanine7 NHS ester
- Sulfo-Cyanine3 maleimide
- Sulfo-Cyanine5 maleimide
- Sulfo-Cyanine7 maleimide
- Sulfo-Cyanine3 azide
- Sulfo-Cyanine5 azide
- Sulfo-Cyanine5.5 azide
- Sulfo-Cyanine7 azide
- Sulfo-Cyanine3 alkyne
- Sulfo-Cyanine5 alkyne
- Sulfo-Cyanine5.5 alkyne
- Sulfo-Cyanine7 alkyne
- Sulfo-Cyanine3 amine
- Sulfo-Cyanine5 amine
- Sulfo-Cyanine5.5 amine
- Sulfo-Cyanine7 amine