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日度归档:2024年8月12日
NISSUI 日本日水培养基 05647 XM-G 琼脂培养基 XM-G Agar 大肠杆菌、大肠菌群用 300g
荧光染料31110 ROX reference dye for qPCR 500 uL
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康宁corning 3752 TGOLD,PCR PLT,96W,FS,CLR,PP,NO 96孔PCR板 透明 全裙边 10个/包
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和光纯药WAKO 019-11941 Active Carbon-impregnated Silica Gel 10g
和光纯药WAKO 019-11941 Active Carbon-impregnated Silica Gel for Dioxins Analysis –
Hampton 96孔坐滴蛋白结晶板–Intelli-Plate
Hampton 96孔坐滴蛋白结晶板–Intelli-Plate
在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton Intelli-Plate 96孔蛋白结晶板的签约代理商,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton Intelli-Plate 96孔蛋白结晶板高品质的产品和服务,欢迎广大新老客户来电咨询。
Intelli-Plate 96 (Art Robbins Instruments)
Applications
Sitting Drop Crystallization 用于蛋白坐滴结晶
Features 特征:
SBS microplate footprint & standard well spacing |
Optically clear wells provide for superior crystal imaging |
Well layout makes crystal harvesting easier |
UV transmissible and low birefringence |
2 well versions available in small and large drop well or two identical shallow drop wells |
3 well versions are ideal for optimizing protein concentration, additive screens, drop ratio and combinatorial experiments |
Rounded reservoir corners to prevent reservoir (MPD) creep |
Description
Art Robbins Instruments has developed the ideal family of plates for crystallography applications: the Intelli-Plate. All versions of the Intelli-Plate have the SBS microplate footprint and standard well spacing and are ideal for manual or automated processing.
The Intelli-Plate 96-2 Low Profile (Art Robbins 102-0001-10 Hampton Research HR3-116 and HR3-117) is a low profile version of the Intelli-Plate 96-2 LVR (Low Volume Reservoir). Reservoir volume: 100 µl max. Drop well volume: 4 µl and 10 µl max. UV transmissible and low birefringence.
The CrystalMation Intelli-Plate 96-3 low-profile crystallization plate (Art Robbins 102-0001-13 Hampton Research HR3-118 and HR3-119) is a low profile version of the Intelli-Plate 96-3 LVR (Low Volume Reservoir). The plate is designed for sitting drop vapor diffusion crystallization experiments. It is constructed from optically clear, UV-transmissible, chemically resistant plastic with superior low birefringence. The low-profile construction allows for higher density in plate storage and imaging systems, maximizing your investment and valuable space. The plate is designed to be a substantial improvement in existing low-profile plates, allowing for superior sealing due to thicker well design and a flat sealing surface. It has been built to the SBS (Society for Biomolecular Screening) standard dimensions with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). It is compatible with automated instrumentation including the CrystalMation line of instruments from Rigaku. The plate features three identical sized sample drop wells per reservoir. The wells are concave depressions along the left side (Y-axis) of the plate and are located on the ledge above the adjacent, flat bottom reagent reservoir. Each well features a round bottom for easy crystal harvesting and can hold up to 1 µl of sample. The multiple well design allows for combinatorial experiments, complex co-crystallization experiments for ligand screening, or for drop concentration variation. The reagent reservoir is typically filled with 50 to 70 µl of reagent. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.
The Intelli-Plate 96-2 LVR (Art Robbins 102-0001-00 Hampton Research HR3-143 and HR3-145) is a Low Volume Reservoir version of the original Intelli-Plate. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.
The Intelli-Plate 96-3 LVR (Art Robbins 102-0001-03 Hampton Research HR3-183 and HR3-185) is a Low Volume Reservoir, 3 drop well version of the Intelli-Plate. Reservoir volume: 100 µl max. Typical reservoir volume 50 µl. Drop well volume: 1 µl max. UV transmissible and low birefringence.
The Intelli-Plate 96-2 Original crystallization plate (Art Robbins 102-0011-00 Hampton Research HR3-297 and HR3-299) is an optically clear plate for sitting drop vapor diffusion crystallization. All wells have standard 9 mm spacing to conform to SBS standards with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). This plate features two locations for sample per reservoir. The sample drop locations are located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. One sample well is located above the second sample well. The top sample well can hold 10 µl or less of sample. The lower sample well can hold 4 µl or less of sample. The reagent reservoir is typically filled with 100 µl of reagent and is capable of holding up to 300 µl of reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and a separation of the reservoirs. This plate is rigid, with virtually no torsional flex and is designed for either manual or automated pipetting. UV transmissible and low birefringence.
The Intelli-Plate Flat Shelf (Art Robbins 102-0001-01 Hampton Research HR8-171 and HR8-172) crystallization plate is a flat drop shelf version of the original Intelli-Plate. Reservoir volume: 300 µl max. No drop well. UV transmissible and low birefringence.
The Intelli-Plate 96-2 Shallow Well (Art Robbins 102-0001-20 Hampton Research HR3-163 and HR3-164) has two identically sized 2 µL reaction wells and a 140 µL screen reservoir. Each of the two identical well features a round bottom shallow well design for easy crystal harvesting and is arranged in an 8 x 12 array. This plate has low birefringence and is UV compatible. This plate is also available in a low profile height (Art Robbins 102-0001-21).
The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).
The height of Intelli-Plate is 0.560 inches. The height of Intelli-Plate Low Profile is 0.315 inches (8.001 mm).
Intelli-Plate 96-3
CAT NO | NAME | DESCRIPTION |
HR3-116 | Intelli-Plate 96-2 Low Profile | 20 plate case |
HR3-117 | Intelli-Plate 96-2 Low Profile | 80 plate case |
HR3-118 | CrystalMation Intelli-Plate 96-3 low-profile | 20 plate case |
HR3-119 | CrystalMation Intelli-Plate 96-3 low-profile | 80 plate case |
HR3-143 | Intelli-Plate 96-2 LVR | 10 plate case |
HR3-145 | Intelli-Plate 96-2 LVR | 40 plate case |
HR3-183 | Intelli-Plate 96-3 LVR | 10 plate case |
HR3-185 | Intelli-Plate 96-3 LVR | 40 plate case |
HR3-297 | Intelli-Plate 96-2 Original | 10 plate case |
HR3-299 | Intelli-Plate 96-2 Original | 40 plate case |
HR8-171 | Intelli-Plate Flat Shelf | 10 plate case |
HR8-172 | Intelli-Plate Flat Shelf | 40 plate case |
HR3-163 | Intelli-Plate 96-2 Shallow Well | 10 plate case |
HR3-164 | Intelli-Plate 96-2 Shallow Well | 40 plate case |
HR3-181 | Intelli-Plate 96-2 Shallow Well Low Profile | 20 plate case |
HR3-182 | Intelli-Plate 96-2 Shallow Well Low Profile | 80 plate case |
Sigma代理 Abcam代理 CST代理 Santa Cruz代理 Biolegend代理 ebioscience代理 Invitrogen代理 millipore代理 BD流式抗体代理 GeneTe x抗体代理 Novus抗体代理 R&D代理 Biovison代理 Jackson代理 MBL抗体代理 ProSpec抗体代理 Bethyl抗体代理 Antibody Revolution抗体代理 Torrey Pines Biolabs代理 Amresco代理 MPbio代理 Laysan bio代理 NANOCS代理 Avanti代理 wako代理 lumiprobe代理(活性荧光染料) NEB酶代理 Roche酶代理 Toyobo酶代理 USP代理 EP代理 Dr代理 TRC代理 TCI代理 Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理 Axygen代理 Falcon代理 NISSUI日水代理 Himedia代理 OXOID代理 BD培养基代理 Ludger代理(糖蛋白分析产品) Eppendorf代理 Labnet代理 标准品代理 抗体代理 酶试剂代理 培养基代理 耗材代理 Elisa试剂盒代理 。
Pall颇尔滤膜 4818 MICROFUNNEL 300SP 0.2UM PK20
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wako适合用于羟甲基化DNA分析5-hydroxymethylcytosine antibody
适合用于羟甲基化DNA分析!5-hydroxymethylcytosine antibody
本品是对应5-hydroxymethylcytosine的单抗,能用于Hydroxymethylated DNA IP (hMeDIP)分析。
免疫动物 |
大鼠 |
类型 |
单克隆 |
交叉性 |
人、鼠等(适用范围广) |
适用 |
hMeDIP、hMeDIP-seq、Dot blotting、 Immunofluorescence |
背景
5-hydroxymethylcytosine是最近发现的修饰碱基,由于oxygenase的运动,从5-methylcytosine(5-mC)被转换成5-hydroxymethylcytosine(5-hmC)。
5-hmC作为第六个碱基在生物体内有什么作用还不明朗,但是,普遍认为它与5-hmC起不同的重要作用。使用普通的硫酸氢钠法不能区别5-mC和5-hmC,所以急需找到可以特异性检测5-hmC的方法。要分别5-mC、5-hmC的序列,利用抗体反应亲和性的技术比较有效,还可以作为特异性的浓缩方法。2
例1
【辨别Dual MeDIP:5-mC和5-hmC】
把基因组DNA用超声波处理,片段化到300 ~ 500 bp,用5-hydroxymethylcytosine antibody (5-hmC抗体:本品)进行羟甲基化DNA IP分析(hMeDIP)。同时对hMeDIP的非结合分层部分,用5-methylcytosine antibody(5-mC抗体)进行甲基化DNA IP分析(MeDIP)。
例2
【和其他公司产品比较】
对5-hydroxymethylcytosine使用2种抗体:“Diagenode公司的单抗(本品)”和“其他公司产品的多抗”进行羟甲基化DNA IP分析(hMeDIP)。在hMeDIP里使用了Diagenode公司的hMeDIP kit。
橙字:Diagenode公司产品单抗
黑字:其他公司单抗
例3
【对单链DNA和双链DNA进行羟甲基化DNA IP分析(hMeDIP)的有效性】
使用5-hydroxymethylcytosine抗体,进行hMeDIP。作为阴性对照,使用小鼠IgG。使用1μg 由hMeDIP kit(Diagenode公司)的GenDNA module制备的ES细胞(E14)的DNA,经BioruptorR进行超声波处理,片段化到300-500bp。
而经免疫沉淀得到的DNA,可使用Sfi1 (splicing factor 1) gene的引物对进行定量PCR的分析。
例4
【用点印迹法时Diagenode公司的5-hmC抗体的特异性】
用5-hmC(1)和5-hmC(2)、C(3)作为各自的底物的PCR产物,经2~200ng(各自含有0.1~10pmol的C碱基)的膜进行点印迹。
在5-hmC抗体里(4μg/ml(稀释倍率1:500))孵育膜。膜暴光30秒。
数据资料
5-hydroxymethylcytosine antibody (diagenode社英語版)
产品编号 |
品名 |
包装 |
313-81061 |
5-hydroxymethylcytosine antibody |
50μg |
刊登的试剂只使用于试验·研究目的,不能作为“医药品”、“食品”、“家庭用品”等使用。
康宁corning 2485 Stripwell,Low Volume,White,Hig 低容量条板 8孔 白色 高吸附 25个/包
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BAMBANKER无血清细胞冻存液
品牌: | Wako |
货号: | 302-14681 |
CAS号: | 无 |
供应商: | 上海金畔生物 |
数量: | 1 |
保存条件: | 2-10℃ |
规格: | 120ml |
BAMBANKER是一种无血清细胞冻存液。可在-80℃长期保存细胞(肿瘤细胞和常规细胞)。
◆产品特性● 即用型细胞冻存液● 无需分步降温,直接使用● 无需稀释● 无需程序降温盒● -80℃长期保存● 无血清◆无血清冻存液的优点 与含血清类型相比,批次间的成分组成差异小,可保持稳定的品质。 不含血清,因此没有因动物源的未知成分和感染物质所产生的影响与风险。 可对无血清驯化细胞进行冷冻,节省再驯化步骤。◆操作流程 1)收集对数生长期的细胞(5×105-1×107个细胞) 2)用1mL该细胞冻存液悬浮细胞,置于冻存管中,不需预冷,直接-80℃冷冻保存,也可用液氮快速冷冻细胞 3)用恒温箱或者水浴锅快速复苏细胞 *冷冻细胞必须处于对数生长期
◆无菌检测** 内毒素:生色底物法 支原体:荧光抗体法 真菌和细菌:依据日本药典 (**:可索取检验证书)BAMBANKER® Direct BAMBANKER Direct是“无血清型”细胞冻存液。◆BAMBANKER® Direct无需离心收集细胞
①不需冻存前预处理,操作简便②不需稀释,直接使用③无需分步降温,直接使用④可快速、长期冻存细胞(-80℃或液氮)⑤不含血清◆BAMBANKER® Direct冻存步骤VS常规冻存步骤 使用本产品无需经过离心等复杂步骤,只需往培养基内添加与培养液等量的BAMBANKER Direct,再分装到冻存管,置于-80℃便可冻存细胞。
◆应用【BAMBANKER使用例】
【低温冻存实验证明以下细胞保存完好】
ES细胞(小鼠)使用实例。 T.Hikichi,et al; Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 2007, 25, 46-53
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产品详细
商品代码其他情报(式样)
|
||
---|---|---|
上海金畔生物科技有限公司
510%2F%3Fc%3D34& ” 510%2F%3Fc%3D34&
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其他,药毒物分析用简易包|简易分析|一般分析纯试剂|试剂|关东化学株式会社
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Bethyl抗体品牌介绍及中国代理商
Bethyl抗体官网 Bethyl抗体中国代理商
Bethyl抗体品牌介绍及中国代理商
品牌介绍
Bethyl公司致力于给研究者提供高品质抗体。主要生产抗体为细胞生物学类。公司为临床和科研提供高品质的抗体生产和服务,拥有世界上最丰富的细胞凋亡产品。如:凯氏半胱氨酸蛋白酶分析试剂盒和试剂,细胞凋亡检测试剂盒及试剂,与细胞凋亡相关的抗体,细胞增殖和细胞毒性检测试剂盒,生长因子,细胞因子和趋化因子,分子生物学试剂盒及试剂等研究产品。
公司信息
联系电话:1-800-338-9579
联系地址:P.O. Box 850 Montgomery, TX 77356
公司官网:http://www.bethyl.com
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BD培养基 216810 10 kg EA Difco Maltose (+), monohydrate 麦芽糖