日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody

日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
Anti Acrolein (ACR) monoclonal antibody.
Suitable for immunohistocheistry. For research use only.
Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Specification

Clone #: 5F6
Antigen: ACR-modified keyhole-lympet hemocyanine
Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
Application: Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
Specificity: Specific for ACR-modified protein (especially FDP-lysine type derivative)
Subclass: Mouse IgG1,kappa
Storage: Less than-20°C

抗丙烯醛单克隆抗体适用于免疫组织化学。
丙烯醛(ACR)是在环境中普遍存在并且通过氧化反应(例如脂质过氧化和髓过氧化物酶催化的氨基酸氧化)内源性形成的代表性致癌醛。 ACR是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。与ACR和赖氨酸残基的反应导致大量的加合物的形成,例如formyl-dehydropiperidino-lysine(FDP-赖氨酸)类型衍生物。
该抗体对ACR修饰的蛋白,特别是FDP-赖氨酸类型衍生物具有特异性。
技术参数
克隆#:5F6;
抗原:ACR修饰的钥孔血蓝蛋白;
形式:冻结(100μg/ mL抗体在含有0.1%NaN3和0.5%BSA的10mM PBS中)。通过蛋白A纯化;
应用:免疫组织化学;
推荐抗体浓度:0.5-1.0μg/ mL对于多聚甲醛固定的组织;
特异性:特定针对ACR修饰的蛋白(特别是FDP-赖氨酸类型衍生物);
亚型:小鼠IgG1,kappa;
储存:小于-20°C

References
1)Protein-bound acrolein: Potential markers for oxidativestress. K.Uchida, M.Kanematsu, K.Sakai, T.Matsuda, N.Hattori,Y.Mizuno, D.Suzuki,T.Miyata, N.Noguchi, E.Niki,T.Osawa
Proc.Natl.Acad.Sci.USA,95,4882-4887(1998)
2)Protein-bound acrolein: A novel markers of oxidativestress in Alzheimer’s Disease. Noel Y. Calingasan, Koji Uchida, and Gary E.Gibson
Journal of Neurochemistry.72(2),751-756(1999)

Product name Code Content
Anti Acrolein monoclonal antibody MAR-020n 20 ug of IgG
MAR-100n 100 ug of IgG

日本 JaICA 老化Anti Acrolein monoclonal antibody
抗丙烯醛(ACR)单克隆抗体 使用使用说明书
技术资料

日本 JaICA代理 Dityrosine (DT) ELISA

日本 JaICA代理 Dityrosine (DT) ELISA
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
Dityrosine (DT) ELISA kit.
Suitable for urine samples. For research use only.
A novel biomarker for protein oxidation

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.
Recently, dityrosine is reported to exist also in urine samples. It is expected that DT may be a novel protein oxdation marker, which is non-invasively detectable. DT ELISA kit is designed for quantitative measurement of DT especially in urine samples.
试剂盒组成:

Samples: Urine
Specifity: Specific to dityrosine (tyrosine dimer)
Assay range: 0.05 – 12 碌mol/L
Measurement: 450 nm
Quantity: 96 wells
Assay time: Overnight and 2 hours
Storage: 2 – 8 掳C. Don’t freeze.
Expiration: Printed on a package


参考文献:

1) Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T:
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
Biochem Biophys Res Commun. 275(1), p11-15 (2000).
2) Hattori Y, Mukaide T, Jiang L, Kotani T, Tsuda H, Mano Y, Sumigama S, Hirayama T, Nagasawa H,Kikkawa F, Toyokuni S:
Catalytic ferrous iron in amniotic fluid as a predictive marker of human maternal-fetal disorders.
J Clin Biochem Nutr 56(1),p57-63(2015) doi: 10.3164/jcbn.14-82
Product name Code Assay range Format Application
Dityrosine (DT) ELISA KDT-010E 0.05 – 12 碌mol/L 96 wells Urine

使用说明书:

Instruction manual (PDF) Technical information

相关产品:

Related products: Anti DT monoclonal antibody

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。
产品类型:
DNA氧化检测产品;
蛋白氧化检测产品;
脂肪氧化检测产品;
抗氧化检测产品。
特性:
高专一性、高灵敏性;
不与RNA损伤产生交叉反应;
易于操作、简单快速;
无需昂贵的设备和样品预处理。
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
产品名称: Anti Di Tyrosine (DT) 单抗
英文名称: Anti Di Tyrosine (DT) monoclonal antibody
产品货号: MDT-020P
产品规格: 20ug
产品产地: 日本
产品商标: JaICA
 

Code Product Name  
KOG-200SE New 8-OHdG Check ELISA kit 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit 96 wells
KPA-050 Test kit for Potential Anti Oxidant (PAO) 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody 20 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.) 1 unit
KDT-010E Dityrosine (DT) ELISA  96 wells
OPNUCP1 Nuclease P1 from Penicillium citrinum 500 units

 

日本Japan Institute for the Control of Aging (JaICA)

日本Japan Institute for the Control of Aging (JaICA)

日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

独特的氧化应激标记物

日本控制衰老研究所从事衰老研究,目的是促进人类健康。该研究所成功研发了检测DNA氧化损伤的试剂盒,并且在这个领域处于领先地位。该研究所目前销售很多关于氧化应激研究方面的产品。这些产品在医学,药学,生物化学和食品方面都有运用。该研究所也提供定制服务。随着步入老龄化社会,预防医学和抗衰老收到越来越多的关注。
JaICA从事试剂盒销售和定制服务。试剂盒部分,研究所研发和生产用于检测氧化应激标记物的试剂盒。单抗和ELISA试剂盒可用于检测活性氧产生的氧化损伤产物。定制服务部分,使用本研究所研发的产品和积累的经验检测尿液和血液样品,检测食品。近来预防医学、抗衰老和功能性食品的关注越来越多,因此全球医院,大学,研究所、食品企业和制药企业分析氧化应激标记物,衰老相关激素和矿物质需求也越来越多。
◆氧化应激标记物概况
 

类型 产品名称 运用 备注
  尿液 血清 组织  
DNA 氧化 8-OHdG   Check ELISA Kit 是(动物) 普遍使用的氧化应激标记物
检测时间:大概3.5小时
8-OHdG   Check ELISA Kit (High Sensitivity) 是(动物) 人血清、组织和培养细胞的更低检测限。
anti-8-Hydroxy-2′-deoxyguanisine   [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反应性
anti-Thymidine   Glycol [TG], mAb (2E8) DNA特异性氧化应激标记物
脂类氧化 Hexanoyl-Lys   [HEL] ELISA Kit n:6 多不饱和脂肪酸早期氧化的新标记物
anti-Hexanoyl-Lys   [HEL], mAb (5F12) 免疫组化,免疫印迹和ELISA
anti-Hydroxy-2-nonenal   [4-HNE], mAb(HNEJ-2) 普遍使用的脂类氧化标志物
anti-Acrolein   [ACR], mAb (5F6) 免疫组化, 免疫印迹 和 ELISA.
anti-Malondialdehyde   [MDA], mAb (1F83) 免疫组化 和 免疫印迹
anti-4-Hydroxy-2-hexenal   [4-HHE], mAb (HHE53) 免疫组化
anti-Crotonaldehyde   [CRA], mAb (82D3) 免疫组化
anti-Methylglyoxal   [MG], mAb (3C) 免疫组化
anti-7-Ketocholesterol   [7-KC], mAb (35A) 胆固醇氧化标记物
蛋白氧化 Dityrosine   [DT] ELISA Kit 蛋白氧化新型标记物。检测时间:过夜 + 1.5小时。
anti-Dityrosine   [DT], mAb (1C3) 免疫组化, 免疫印迹 和 ELISA.
anti-Dibromo-tyrosine   [DiBrY], mAb (3A5) 炎症相关的蛋白氧化标记物
抗氧化检测 Total   Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水总抗氧化检测,能够疏水和亲水的抗氧化剂。

 

日本老化JaICA中国代理及JaICA品牌介绍

日本老化JaICA中国代理及JaICA品牌介绍

Japan Institute for the Control of Aging (JaICA)

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理

日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。
产品类型:
1. DNA氧化检测产品;
2. 蛋白氧化检测产品;
3. 脂肪氧化检测产品;
4. 抗氧化检测产品。
特性:
1. 高专一性、高灵敏性;
2. 不与RNA损伤产生交叉反应;
3. 易于操作、简单快速;
4. 无需昂贵的设备和样品预处理。

日本Jaica
The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.

独特的氧化应激标记物

日本控制衰老研究所从事衰老研究,目的是促进人类健康。该研究所成功研发了检测DNA氧化损伤的试剂盒,并且在这个领域处于领先地位。该研究所目前销售很多关于氧化应激研究方面的产品。这些产品在医学,药学,生物化学和食品方面都有运用。该研究所也提供定制服务。随着步入老龄化社会,预防医学和抗衰老收到越来越多的关注。
JaICA从事试剂盒销售和定制服务。试剂盒部分,研究所研发和生产用于检测氧化应激标记物的试剂盒。单抗和ELISA试剂盒可用于检测活性氧产生的氧化损伤产物。定制服务部分,使用本研究所研发的产品和积累的经验检测尿液和血液样品,检测食品。近来预防医学、抗衰老和功能性食品的关注越来越多,因此全球医院,大学,研究所、食品企业和制药企业分析氧化应激标记物,衰老相关激素和矿物质需求也越来越多。
◆氧化应激标记物概况

类型 产品名称 运用 备注
尿液 血清 组织
DNA 氧化 8-OHdG   Check ELISA Kit 是(动物) 普遍使用的氧化应激标记物
检测时间:大概3.5小时
8-OHdG   Check ELISA Kit (High Sensitivity) 是(动物) 人血清、组织和培养细胞的更低检测限。
anti-8-Hydroxy-2′-deoxyguanisine   [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反应性
anti-Thymidine   Glycol [TG], mAb (2E8) DNA特异性氧化应激标记物
脂类氧化 Hexanoyl-Lys   [HEL] ELISA Kit n:6 多不饱和脂肪酸早期氧化的新标记物
anti-Hexanoyl-Lys   [HEL], mAb (5F12) 免疫组化,免疫印迹和ELISA
anti-Hydroxy-2-nonenal   [4-HNE], mAb(HNEJ-2) 普遍使用的脂类氧化标志物
anti-Acrolein   [ACR], mAb (5F6) 免疫组化, 免疫印迹 和 ELISA.
anti-Malondialdehyde   [MDA], mAb (1F83) 免疫组化 和 免疫印迹
anti-4-Hydroxy-2-hexenal   [4-HHE], mAb (HHE53) 免疫组化
anti-Crotonaldehyde   [CRA], mAb (82D3) 免疫组化
anti-Methylglyoxal   [MG], mAb (3C) 免疫组化
anti-7-Ketocholesterol   [7-KC], mAb (35A) 胆固醇氧化标记物
蛋白氧化 Dityrosine   [DT] ELISA Kit 蛋白氧化新型标记物。检测时间:过夜 + 1.5小时。
anti-Dityrosine   [DT], mAb (1C3) 免疫组化, 免疫印迹 和 ELISA.
anti-Dibromo-tyrosine   [DiBrY], mAb (3A5) 炎症相关的蛋白氧化标记物
抗氧化检测 Total   Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水总抗氧化检测,能够疏水和亲水的抗氧化剂。

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗4-羟基壬烯醛单克隆抗体
Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNEJ-2)
货号:MHN-100P
品牌:JaICA
规格: 100µg
产品说明 / Product Overview:
Anti 4-HNE monoclonal antibody (HNEJ-2)

Structure of 4-hydroxy-2-nonenal
 
脂质氧化标志物
适用于免疫组织化学和免疫印迹.(仅供研究使用)
关于4-羟基壬烯醛(4-hydroxy-2-nonenal; 4-HNE)
活性氧(ROS)参与活体内的脂质过氧化,膜脂质是ROS的主要靶标之一。 ROS在过氧化过程中形成各种醛。 4-羟基-2-壬烯醛(4-HNE)是一种α,β不饱和醛,可以通过omega-6不饱和脂肪酸(例如亚油酸和花生四烯酸)的过氧化形成。 特别需要指出的是,据报道在活体内形成的4-HNE源自磷脂结合的花生四烯酸。 4-HNE可能是脂质过氧化最可靠的生物标志物之一。
大肠癌细胞的高倍视图:腺癌(箭头所示)和非肿瘤性上皮细胞(star x240)。
(S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

技术参数
克隆号#:HNEJ-2;
免疫原:4-HNE修饰的钥孔血蓝蛋白(KLH);
亚基:小鼠IgG1(kappa);制备为腹水,并纯化硫酸铵;
性状:冻干粉(含20μg或100μg IgG);
特异性:该抗体对于用其它醛(如2-壬烯醛,2-己烯醛,1-己醛,4-羟基-2-己烯醛,甲醛或戊二醛)处理的蛋白质显示出几乎可以忽略的反应性;
应用:免疫组织化学和免疫印迹;
储存:-20°C以下5年;运输时,室温下至少可稳定保持7天。

Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 碌g or 100 碌g of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20掳C for 5 years. When transport, stable at least 7 days at room temperature.

References

Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
Product name Code Content
Anti 4-HNE monoclobal antibody MHN-020P 20 ug of IgG/ vial
MHN-100P 100 ug of IgG/ vial

使用说明书
免疫组织化学操作方法
技术资料

日本 JaICA代理 Anti HEL monoclonal antibody

日本 JaICA代理 Anti HEL monoclonal antibody
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
适用于免疫组织化学和ELISA。
Anti hexanoyl-Lysine adduct monoclonal antibody.
Suitable for immunohistocheistry and ELISA. For research use only.
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
由活性氧(ROS)引起的脂质氧化损伤在一些疾病,细胞功能损害和衰老中起重要作用。醛类(如丙二醛/MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一,但最近在脂质过氧化的早期阶段,发现13-hydroperoxyoctadecanoic acid(13-HPODE)与蛋白质1)共价结合。
己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基, HEL通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)氧化修饰形成。
抗己酰-赖氨酸(HEL)单克隆抗体对于含有HEL的蛋白质和肽是特异的,适用于通过免疫组织化学和免疫印迹的HEL检测。
A new biomarker for lipid peroxidation   脂质过氧化新的生物标志物


Cisplatin-treated rat kidney. 顺铂处理的大鼠肾脏
(Dr. Sugiyama, Tottori Univ.)  (Sugiyama博士)

Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)
HEL在人类动脉粥样硬化病变的检测  (Naito博士)

Clone #: 5F12
Subclass: IgG1 kappa
Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH)
Specificity: Specific to Hexanoyl-Lys adducts.
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 碌g/mL of IgG.
Application: Immunohistochemistry
(Recommended antibody concentration is 2 碌g/mL on paraformaldehyde fixed tissue), and ELISA
Storage: Less than -20C

• 技术参数
克隆#:5F12;
亚基:IgG1 kappa;
抗原:N epsilon己酰-钥孔血蓝蛋白(HEL-KLH);
特异性:特异于己酰-赖氨酸加合物;
形式:冻干粉,100μg/mL(200μL),PBS(pH7.4),包含5%蔗糖,1% BSA;
应用:免疫组织化学(多聚甲醛固定的组织推荐抗体浓度为2μg/mL)和ELISA;
储存:低于-20°C
参考文献:

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.
4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).
5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.

产品信息:

Product name Code Specifications Application NOTICE
Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20ug of IgG Immunohistochemistry Current product
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20ug of IgG Immunohistochemistry Discontinued

使用说明书
免疫组织化学方法
技术信息
克隆#5F12和5H4的比较

Instruction manual (PDF) Immunohistochemical procedure Technical information
Comparison of clone 5F12 and 5H4 (PDF)

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody
中文名称:抗丙二醛单克隆抗体
英文名称:Anti Malondialdehyde(MDA) monoclonal antibody
货号:MMD-030n
规格:30ug
Anti MDA monoclonal antibody  脂质过氧化生物标志物
适用于免疫组化(仅供研究使用)
About malondialdehyde (MDA)
Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

关于丙二醛(MDA)
丙二醛(MDA)是衍生自脂质过氧化的主要醛之一。 MDA是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。 与MDA和赖氨酸残基的反应导致众多数量的加合物的形成,例如二氢吡啶-赖氨酸(DHP-赖氨酸)型衍生物。 该单克隆抗体对MDA修饰的蛋白质,特别是DHP-赖氨酸型衍生物是特异性的。
Specifications

Clone #: 1F83
Antigen: MDA-modified keyhole-lympet hemocyanine.
Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
Application: Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
Specificity: MSpecific for MDA-modified protein (especially DHP-lysine).
Subclass: Mouse IgG2a(lambda)
Storage: Less than -20掳C


Specificity of anti MDA antibody.

Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
Dr. N Shibata, Tokyo Women’s Medical University.
技术参数
克隆号#:1F83;
抗原:MDA修饰的钥孔血蓝蛋白(KLH);
性状:冻结(100μg/mL抗体在含有0.1%NaN3和0.5% BSA的10mM PBS中),经过蛋白A纯化;
应用:免疫组织化学。对多聚甲醛固定的组织推荐抗体浓度为0.5-1.0μg/mL;
特异性:特异性针对MDA修饰的蛋白(特别是DHP-赖氨酸)。
亚基:小鼠IgG2a(lambda)
储存:小于-20℃
其他相关产品:
·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
·  抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
·  己酰-赖氨酸加合物ELISA试剂盒 Hexanoyl-Lysine adduct(HEL) ELISA Kit
References 参考文献

1) Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine.
S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida.
J.Lipid Res. 42, p1187-1196 (2001)
2) Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.
Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E
Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody技术资料:http://www.jaica.com/e/technical_forum_nof.html#MDA
日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody 说明书:
http://www.jaica.com/e/pdf/mda_ab_manual.pdf

日本JaICA试剂盒产品目录

日本JaICA试剂盒产品目录
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

序号 详细名称 品牌 规格
KOG-200SE New 8-OHdG Check ELISA kit/8羟基脱氧茑苷ELISA 试剂盒 JaICA 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) JaICA 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit/高灵敏 8羟基脱氧茑苷ELISA 试剂盒 JaICA 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody/8羟基脱氧茑苷 单克隆抗体 JaICA 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody/8羟基脱氧茑苷 单克隆抗体 JaICA 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody/4羟基壬烯酸单抗 JaICA 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody/4羟基壬烯酸单抗 JaICA 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody/二溴络氨酸单抗 JaICA 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody/抗胸苷乙二醇单克隆抗体 JaICA 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody/二络氨酸抗体 JaICA 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12)/乙酰赖氨酸单克隆抗 JaICA 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit/乙酰赖氨酸加合物ELISA试剂盒 JaICA 96 wells
KPA-050E Test kit for Potential Anti Oxidant (PAO)/总抗氧化能力ELISA试剂盒 JaICA 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody/丙二醛单克隆 JaICA 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody/炳烯醛单克隆 JaICA 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody/炳烯醛单克隆 JaICA 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody/4羟基 2烯醛单抗 JaICA 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody/抗巴斗醛单抗 JaICA 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody/甲基乙二醛单抗 JaICA 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基胆固醇单抗 JaICA 20 micro gram
MKC-100n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基胆固醇单抗 JaICA 100 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.)/血清预处理超滤装置(MW切断10kDa,Pall公司) JaICA 1 unit
KDT-010E Dityrosine (DT) ELISA/双络氨酸ELISA试剂盒 JaICA  96 wells

 

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本JaICA Test kit for Potential Anti Oxidant (PAO)
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.
Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25掳C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 
Content of this kit: 试剂盒组成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and L Galli
Clinical Immunology 100(1),p82-86 (2001)
Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)   说明书  技术资料

Instruction manual Technical information